Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

Article Title: Immunosenescence and cytomegalovirus-associated immune signatures on severe acute respiratory syndrome coronavirus 2 booster responses

doi: 10.1093/gerona/glag095

Figure Lengend Snippet: Humoral response to the booster vaccine dose. Responses in the overall cohort (A) and within sub-cohorts defined by age (B), sex (C), prior SARS-CoV-2 infection (D), and CMV serostatus (E). * p < .05; ** p < .01; **** p < .0001. (paired t -test or Wilcoxon signed-rank test following the Shapiro–Wilk test). (F) Partial correlations (Pearson’s r ) between SARS-CoV-2 anti-RBD IgG at T0 and T1. Residuals were derived from linear regression models adjusted for age, sex, CMV serostatus, and prior SARS-CoV-2 infection. Degrees of freedom = 41. T0 = pre-boost; T1 = post-boost.

Article Snippet: In this study, we evaluated the effect of a fourth Pfizer SARS-CoV-2 booster dose on humoral and cellular immune responses in a healthy population, considering age, sex, prior SARS-CoV-2 infection, and CMV serostatus, and the impact of immunosenescence.

Techniques: Infection, Derivative Assay

Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

Article Title: Immunosenescence and cytomegalovirus-associated immune signatures on severe acute respiratory syndrome coronavirus 2 booster responses

doi: 10.1093/gerona/glag095

Figure Lengend Snippet: CD4+ and CD8+ T-cell responses to SARS-CoV-2 in the overall cohort. (A) Cellular responses. IFNt: total IFN-γ+ cells (IFN-γ+TNF-α+/−); TNFt: total TNF-α+ cells (TNF-α+IFN-γ+/−); mono-functional cells (IFNm: IFN-γ+TNF-α−; TNFm: TNF-α+IFN-γ−); and bifunctional cells (bi: IFN-γ+TNF-α+). (B–G) CD4+ T-cell responses to SARS-CoV-2 in the overall cohort. (H–M) CD8+ T-cell responses to SARS-CoV-2 in the overall cohort. Circles denote pre-boost (T0) responses and triangles denote post-boost (T1) responses. Lines connect paired observations from the same individual. * p < .05; ** p < .01; *** p < .001 (Wilcoxon signed-rank test following the Shapiro–Wilk test).

Article Snippet: In this study, we evaluated the effect of a fourth Pfizer SARS-CoV-2 booster dose on humoral and cellular immune responses in a healthy population, considering age, sex, prior SARS-CoV-2 infection, and CMV serostatus, and the impact of immunosenescence.

Techniques: Functional Assay

Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

Article Title: Immunosenescence and cytomegalovirus-associated immune signatures on severe acute respiratory syndrome coronavirus 2 booster responses

doi: 10.1093/gerona/glag095

Figure Lengend Snippet: Effect of covariates on CD4+ T-cell cytokine outputs following ANCOVA. (A) Effect of sex and prior SARS-CoV-2 infection (COVID) on changes (Δ) in CD4+ IFNt and IFNm responses. (B) Effect of sex and age on CD4+ T-cell responses after the booster (T1), including cytokine percentages and their iMFI. For each cytokine output, symbols represent estimated marginal means for the sub-cohort groups, with 95% confidence intervals (CIs) shown as horizontal lines. p -values in the embedded table indicate the effect of each covariate on cytokine outputs and were obtained from Type III ANOVA (sums of squares). ns = not significant ( p > .05).

Article Snippet: In this study, we evaluated the effect of a fourth Pfizer SARS-CoV-2 booster dose on humoral and cellular immune responses in a healthy population, considering age, sex, prior SARS-CoV-2 infection, and CMV serostatus, and the impact of immunosenescence.

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: Longitudinal evaluation of T-cell responses to Pfizer-BioNTech and Janssen SARS-CoV-2 vaccines as boosters in Ghanaian adults

doi: 10.3389/fimmu.2025.1643083

Figure Lengend Snippet: Longitudinal analysis of IFN-γ, TNF-α, IL-1β & IL-6 responses (pg/ml) in individuals who received the Janssen (A–D) or Pfizer (E–H) booster vaccines. Friedman’s test and Dunn’s multiple comparisons were used for across timepoints comparison within the Janssen and Pfizer groups. M0 = Samples taken before booster administration; M3, M6, and M9 = Samples taken at 3, 6, and 9 months after booster vaccine administration. Data points represent the median and the error bars, the minimum to maximum range.

Article Snippet: Janssen vs Pfizer booster vaccine , 2.11251 , 2.14336 , 0.986 , 0.3243 , .

Techniques: Vaccines, Comparison

Journal: Frontiers in Immunology

Article Title: Longitudinal evaluation of T-cell responses to Pfizer-BioNTech and Janssen SARS-CoV-2 vaccines as boosters in Ghanaian adults

doi: 10.3389/fimmu.2025.1643083

Figure Lengend Snippet: Longitudinal analysis of IL-2, IL-12, and IL-17 responses (pg/ml) in individuals who received Janssen (A–C) or Pfizer (D–F) booster vaccines. Friedman’s test and Dunn’s multiple comparisons were used for comparisons across time points in the Janssen and Pfizer recipients. M0 = Samples taken before booster administration; M3, M6, and M9 = Samples taken at 3, 6, and 9 months after booster administration. Data points represent the median and the error bars, the minimum to maximum range.

Article Snippet: Janssen vs Pfizer booster vaccine , 2.11251 , 2.14336 , 0.986 , 0.3243 , .

Techniques: Vaccines

Journal: Frontiers in Immunology

Article Title: Longitudinal evaluation of T-cell responses to Pfizer-BioNTech and Janssen SARS-CoV-2 vaccines as boosters in Ghanaian adults

doi: 10.3389/fimmu.2025.1643083

Figure Lengend Snippet: Longitudinal analysis of IL-4 and IL-10 responses (pg/ml) in individuals who received the Janssen (A, B) or Pfizer (C, D) booster vaccines. Friedman’s test, and Dunn’s multiple comparisons were used for across time point comparisons in the Janssen and Pfizer recipients. M0 = Samples taken before booster administration, M3, M6 & M9 = Samples taken at months 3, 6, and 9 post booster administration. Data points represent the median and the error bars, the minimum to maximum range.

Article Snippet: Janssen vs Pfizer booster vaccine , 2.11251 , 2.14336 , 0.986 , 0.3243 , .

Techniques: Vaccines

Journal: Frontiers in Immunology

Article Title: Longitudinal evaluation of T-cell responses to Pfizer-BioNTech and Janssen SARS-CoV-2 vaccines as boosters in Ghanaian adults

doi: 10.3389/fimmu.2025.1643083

Figure Lengend Snippet: Magnitude of IFN-γ (A) , IL-1β (B) , TNF-α (C) and IL-6 (D) responses between Janssen and Pfizer booster vaccinated individuals. The data are represented as box and whisker plots; p-values were calculated at each timepoint using the Mann-Whitney U-test between the Janssen and Pfizer groups with no significant differences reported (p>0.05). Month 0 = Samples taken before booster administration, Month 3, 6 & 9 = Samples taken at months 3, 6, and 9 post booster administration. White horizontal lines represent the median while the whisker represents the minimum to maximum range.

Article Snippet: Janssen vs Pfizer booster vaccine , 2.11251 , 2.14336 , 0.986 , 0.3243 , .

Techniques: Whisker Assay, MANN-WHITNEY